KLEB-GAP webinar, September 2021
Online, September 6-8 2021
KLEB-GAP webinar, September 6-8 2021
WP3: Geno- and phenotype associations in AMR and virulence prediction.
09:15 - 09:20: Welcome to KLEB-GAP Webinar – Arnfinn Sundsfjord
09:20 - 09:30: Overview of WP3 – Ørjan Samuelsen
09:30 - 09:50: Human KLEB-GAP Kp collections – Marit Hetland
09:50 - 10:10: Update on Kp pathogenesis – Jose Bengoechea
10:10 - 10:20: Break
10:20 - 10:40: Preliminary results from virulence assay screening – Jose Bengoechea
10:40 - 11:00: Discussion/comments from SAB
11:00 - 11:10: Genotype-phenotype AMR – Ørjan Samuelsen
11:10 - 11:30: Kleborate and AMR prediction – Kat Holt
11:30 - 11:45: Siderophores & Cefiderocol: a small pilot study – Ken Lindstedt
11:45 - 12:00: Discussion/comments from SAB
WP4: Phage therapy against MDR-Kp.
13:00 - 13:30: Overview of WP4 – Christian Giske
13:30 - 14:00: Insights from animal studies using phages – Martha Clokie
14:00 - 14:15: Break
14:15 - 14:45: Characterization of Klebsiella pneumoniae isolates by predicted serotype, CRISPR-Cas protospacer targets, and phage sensitivity – Mia Winkler
14:45 - 15:10: Update from Stim – Cyril Franzén
15:10 - 15:35: Respiratory animal model – José Bengoechea
15:35 - 16:00: Discussion/concluding remarks
WP1: Determine the presence, population structure, and pan-genome of Kp in human, animal, and marine reservoirs to elucidate potential eco-adaptation and crosstalk between reservoirs.
09:00 - 09:10: Welcome and overview of WP1 – Iren Löhr
09:10 - 09:30: News in Kp nomenclature – Sylvain Brissè
09:30 - 09:50: Kleborate update – Kat Holt
09:50 - 10:10: Update from the animal niche – Håkon Kaspersen
10:10 - 10:30: Update from the marine niche – Fredrik Håkonsholm
10:30 - 10:40: Break
10:40 - 11:00: Kp across niches in KLEB-GAP: an overview – Marit Hetland
11:00 - 11:20: Discussion and comments from SAB
11:20 - 11:40: The SpARK study: Kp transmission modelling across niches – Ed Feil
11:40 - 12:00: Discussion and comments from SAB
WP2: Exploring whole metagenomic sequencing (WMS) for detection and indepth analysis of K. pneumoniae (Kp) population genomics in selected reservoirs.
13:10 - 13:30: Overview bioinformatic pipeline for Kp-fecal metagenomics – Dorota Buczek
13:30 - 13:50: Detection of Kp human gut colonisation: a comparison of culture, qPCR and whole metagenomic sequencing methods and brief introduction to the longitudinal human Kp-study – Ken Lindstedt
13:50 - 14:10: Discussions and comments from SAB
14:10 - 14:20: Break
14:20 - 14:50: Plan/discussion on faecal metagenomics in farm animals - Håkon Kaspersen and Marianne Sunde
14:50 - 15:10: Unravelling Klebsiella-ST diversity in bacterial sweeps from SCAI-agar – Dorota
15:10 - 15:25: Kp-enrichment in WMS using MyBaits RNA-probes – Ken Lindstedt
15:25 - 15:40: Alternative Kp-enrichment methods in WMS – Kat Holt
15:40 - 16:00: Discussions/comments from SAB
KLEB-GAP partner meeting for summary and future planning
1. Brief summary from each WP and specific comments from SAB during each WPwebinar
2. Future meeting activities
3. Other businesses
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